BIEMANS, RALPH LEON,DUVIVIER, PIERRE,GAVARD, OLLIVIER FRANCIS NICOLAS
申请号：
CA2828787
公开号：
CA2828787A1
申请日:
2012.03.05
申请国别(地区):
CA
年份:
2012
代理人:
摘要：
The present invention process a process for conjugation of an antigen comprising the steps of a) activating the antigen to form an activated antigen； b) reacting the activated antigen and a carrier protein or a linker to form an imine group linking the activated antigen to the carrier protein or the linker； and c) reducing the imine group using a reducing agent comprising a triacetoxyborohydride moeity to form a conjugated antigen or an antigen-linker； and optionally d) reacting the antigen linker with a carrier protein to form a conjugated antigen； optionally wherein the antigen originates from Streptococcus pneumoniae, Haemophilus influenzae, Neisseria meningitidis, Staphylococcus aureus, Enterococcus faecium, Enterococcus faecalis, Salmonella Vi, or Staphylococcus epidermidis, optionally further comprising a step a') of lyophilising the activated antigen and a carrier protein followed by reconstitution in DMSO or DMF.
