The present invention relates to a recombinant adenoviral vector for generating immunity against enterovirus infection. In one embodiment, the recombinant adenoviral vector of the invention comprises an expression cassette encoding a P1 protein and a 3CD protease of an enterovirus. In another embodiment, the recombinant adenoviral vector of the invention comprises an expression cassette encoding a 3C protease or a 3CD protease of an enterovirus. The present invention also relates to a vaccine composition comprising the recombinant adenoviral vector as described. A method of inducing an immune response in a subject against enterovirus infection using the recombinant adenoviral vector and the vaccine composition is provided. Further provided is a method for producing virus like particles of an enterovirus by expressing the adenoviral vector as described herein in mammalian cells.本發明關於一種重組腺病毒載體，可用於產生免疫力以對抗腸病毒感染。於一具體實施例中，本發明之重組腺病毒載體包含一表現卡匣，其編碼腸病毒之P1蛋白及3CD蛋白酶。於另一具體實施例中，本發明重組腺病毒載體包含一表現卡匣，其編碼腸病毒之3C蛋白酶或3CD蛋白酶。本發明亦關於一種疫苗組合物，包含所述之重組腺病毒載體。本發明提供一種使用該重組腺病毒載體及疫苗組合物以誘發個體產生免疫反應，對抗腸病毒感染的方法。進一步提供一種產生腸病毒之類病毒顆粒之方法，係藉由表現本文所述之腺病毒載體於哺乳動物細胞中。
