A virtual reality-based ophthalmic inspection system includes a wearable unit, an electronic unit, and at least one detector. The wearable unit is available for an inspected object to wear the wearable unit on head. The electronic unit is assembled with the wearable unit and has a left-eye display zone and a right-eye display zone. A first visual acuity of the inspected object is identified according to a first eyesight information. The electronic unit performs a visual correction confirmation process, the at least one detector detects a condition value of corresponding the right eye and/or the left eye, and the electronic unit answers a first comparison result after comparing the condition value with a threshold value.