Hugo Lavoie,Malha Sahmi,Marc Therrien,Thanashan Rajakulendran,Frank Sicheri
申请号：
US14135934
公开号：
US09464313B2
申请日:
2013.12.20
申请国别(地区):
US
年份:
2016
代理人:
摘要：
Disclosed herein are biosensors useful for detecting the dimerization of RAF and/or KSR polypeptides. These biosensors comprise fusion proteins comprising RAF and/or KSR proteins fused to bioluminescent or fluorescent proteins. Also disclosed are methods of using the biosensors to detect and measure the dimerization of RAF/RAF and RAF/KSR polypeptides by resonance energy transfer such as BRET or FRET, for example to screen for inhibitors of dimerization.
