LAVOIE, HUGO,SAHMI, MALHA,THERRIEN, MARC,RAJAKULENDRAN, THANASHAN,SICHERI, FRANK
申请号：
CA2768042
公开号：
CA2768042C
申请日:
2010.07.23
申请国别(地区):
CA
年份:
2017
代理人:
摘要：
Disclosed herein are mutated RAF and KSR nucleic acids and polypeptides. Alsodisclosed are methods of using the mutated RAF and KSR to inhibit thedimerizationof RAF/RAF and RAF/KSR. Also disclosed are methods of using the mutated RAFand KSR to screen for inhibitors of dimerization. Also disclosed areBioluminescenceResonance Energy Transfer (BRET)-based biosensors comprising cells expressingRAF polypeptides fused to a BRET donor or acceptor, as well as methods usingthesebiosensors for monitoring RAF dimerization.
