The current invention is directed towards a rapid, reproducible test for the fungal virulence factors and associated affected compounds so that patients at risk for autism, cerebral palsy and other human diseases can be quickly identified, treated and possibly prevented. This includes a multitude of test protocols for both the mycotoxin gliotoxin and its relationship with glutathione- and relates the two molecules by a novel paradigm known as the Glutathione-Gliotoxin Index-(GGI) or Disease-Disorder Susceptibility Index-D/DSI, which indicates the Autism Susceptibility Index-(ASI). One testing device and protocol includes a cellular phone application with modification to test for susceptibility to autism and other disease states. Of particular relevance for autism is the role of glutathione depletion by gliotoxin, then by the anti-pyretic acetaminophen in the peri-vaccination period, which each result in oxidative stress and metal intoxication, by disrupting the metallothionein system, amongst other biochemical pathways and numerous enzyme systems.
