The present invention provides a method for producing a nervous system cell or nerve tissue comprising the following steps (1) to (3): (1) pluripotent stem cells in the absence of feeder cells, 1) TGFβ family signals A first step of culturing in a medium containing a transmission pathway inhibitor and / or a sonic hedgehog signal transduction pathway agent, and 2) an undifferentiated maintenance factor, (2) suspension culture of the cells obtained in the first step, The second step of forming cell aggregates, and (3) the aggregate obtained in the second step is suspended in the presence or absence of a differentiation-inducing factor to produce a coagulation containing neural cells or neural tissue. Third step to obtain a collection.本発明は、下記工程(1)～(3)を含む、神経系細胞又は神経組織の製造方法を提供する：（1）多能性幹細胞を、フィーダー細胞非存在下で、1）TGFβファミリーシグナル伝達経路阻害物質及び／又はソニック・ヘッジホッグシグナル伝達経路作用物質、並びに2）未分化維持因子を含む培地で培養する第一工程、（2）第一工程で得られた細胞を浮遊培養し、細胞の凝集体を形成させる第二工程、及び（3）第二工程で得られた凝集体を、分化誘導因子の存在下もしくは非存在下に浮遊培養し、神経系細胞もしくは神経組織を含む凝集体を得る第三工程。
