PROBLEM TO BE SOLVED: To provide factors which mobilize stem cells in functional regeneration promotion of a damaged tissue, to a damaged site.SOLUTION: The present invention relates to: 1) a large amount of bone marrow-derived cells are mobilized to grafted skin； 2) the mobilized bone marrow-derived cells differentiate into any of dermal fibroblasts, adipocytes, muscle cells, vascular endothelial cells and epidermal keratinocytes in the grafted skin piece, and in the mobilized bone marrow-derived cells, bone marrow-derived mesenchymal stem cells are included； and 3) factors released from a necrosed tissue of the grafted skin which mobilize the marrow-derived mesenchymal stem cell to the grafted skin piece from peripheral blood are isolated and identified. The isolated and identified factors which promote migration of mesenchymal stem cells separated and cultured from a purified bone marrow are S100A8 and S100A9.SELECTED DRAWING: None【課題】損傷組織の機能的再生促進における幹細胞を損傷部位に動員する因子の提供。【解決手段】１）骨髄由来細胞が移植皮膚内に大量に動員されること、２）動員された骨髄由来細胞は移植皮膚片内で真皮線維芽細胞、脂肪細胞、筋肉細胞、血管内皮細胞、表皮角化細胞、のいずれにも分化しており、動員された骨髄由来細胞の中に骨髄由来間葉系幹細胞が含まれていること、３）骨髄由来間葉系幹細胞を末梢血から植皮片に動員している移植皮膚の壊死組織から放出された因子を単離・同定する。精製した骨髄から分離・培養した間葉系幹細胞の遊走を促進する単離・同定された因子はS100A8、S100A9である。【選択図】なし
