Qing Huang;Barbara Perilli-Palmer;Min Wan;Robert K. Hickman;Cheryl L. Weed;Scott T. Ennis
发明人:
Robert K. Hickman,Qing Huang,Cheryl L. Weed,Scott T. Ennis,Barbara Perilli-Palmer,Min Wan
申请号：
US12582506
公开号：
US08895709B2
申请日:
2009.10.20
申请国别(地区):
US
年份:
2014
代理人:
摘要：
Disclosed herein are methods for the isolation and purification of antibodies wherein the use of an affinity chromatographic step results in an antibody composition sufficiently pure for pharmaceutical uses. The methods described herein comprise pH viral reduction/inactivation, ultrafiltration/diafiltration, affinity chromatography, preferably Protein A affinity, ion exchange chromatography, and hydrophobic chromatography. Further, the present invention is directed toward pharmaceutical compositions comprising one or more antibodies of the present invention.
