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Isolation and purification of anti-IL-13 antibodies using protein A affinity chromatography
专利权人:
Robert K. Hickman
发明人:
Robert K. Hickman
申请号:
US12908502
公开号:
US08491904B2
申请日:
2010.10.20
申请国别(地区):
US
年份:
2013
代理人:
摘要:
Disclosed herein are methods for the isolation and purification of anti-IL-13 antibodies wherein the use of an affinity chromatographic step results in an antibody composition sufficiently pure for pharmaceutical uses. The methods described herein comprise pH viral reduction/inactivation, ultrafiltration/diafiltration, affinity chromatography (e.g., Protein A affinity chromatography), ion exchange chromatography, and hydrophobic chromatography. Further, the present invention is directed toward pharmaceutical compositions comprising one or more antibodies of the present invention.
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