Giovanni Mistrello,Daniela Roncarolo,Dario Zanoni,Stefania Zanotta,Paolo Falagiani
申请号：
US14691003
公开号：
US10526365B2
申请日:
2015.04.20
申请国别(地区):
US
年份:
2020
代理人:
摘要：
A method of making and a modified allergen having reduced allergenicity and preserved immunogenicity compared to corresponding native allergenic material. The preserved immunogenicity is elicited by a IgG-mediated antibody response, all or part of the primary amine groups of the lysine residues of the native allergenic material are functionalized with carbamoyl or thiocarbamoyl moieties, and all or part of the primary amine groups of the arginine residues of the carbamoyl- or thiocarbamoyl functionalized allergenic material are further functionalized with dialdehyde or diketal moieties. The raw allergenic material is selected from the group consisting of DP mites extract, Der p1, ovalbumin and Lipid Transfer Protein (LTP), the average percentage of modified primary amine groups of the lysine ranges between 75% and 100%； and the average percentage of the substituted arginine residues ranges between 25% and 10%.
