The Government of The United States of America, as represented by The Secretary, Department of Health and Human Services;Royal Cornwall Hospital Trust
发明人:
申请号：
EP12734409.1
公开号：
EP2663328A1
申请日:
2012.01.10
申请国别(地区):
EP
年份:
2013
代理人:
摘要：
The invention relates to the discovery of an HEV strain from a chronically infected patient. The virus grow unusually well in numerous cell cultures. Thus, the invention provides cell cultures, vectors, and vaccine compositions based on the virus. The invention relates, in part, on the identification of a new strain of HEV genotype 3 virus. Strain Kernow-C1 (genotype 3) of HEV, which was isolated from a chronically infected patient, was used to identify human, pig and deer cell lines permissive for infection. Adaptation of the Kernow-C 1 strain to growth in human hepatoma cells selected for a rare virus recombinant that contained an insertion of 174 ribonucleotides (58 amino acids) of a human ribosomal protein gene and additional mutations.
