ROYAL CORNWALL HOSPITAL TRUST; AS REPRESENTED BY THE SECRETARY;THE GOVERNMENT OF THE UNITED STATES OF AMERICA; DEPARTMENT OF HEALTH AND HUMAN SERVICES;THE GOVERNMENT OF THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES
发明人:
EMERSON, SUZANNE U.,SHUKLA, PRIYANKA,NGUYEN, HANH T.,PURCELL, ROBERT H.,DALTON, HARRY R.,BENDALL, RICHARD P.
申请号：
EP12734409
公开号：
EP2663328A4
申请日:
2012.01.10
申请国别(地区):
EP
年份:
2014
代理人:
摘要：
The invention relates to the discovery of an HEV strain from a chronically infected patient. The virus grow unusually well in numerous cell cultures. Thus, the invention provides cell cultures, vectors, and vaccine compositions based on the virus. The invention relates, in part, on the identification of a new strain of HEV genotype 3 virus. Strain Kernow-C1 (genotype 3) of HEV, which was isolated from a chronically infected patient, was used to identify human, pig and deer cell lines permissive for infection. Adaptation of the Kernow-C 1 strain to growth in human hepatoma cells selected for a rare virus recombinant that contained an insertion of 174 ribonucleotides (58 amino acids) of a human ribosomal protein gene and additional mutations.
