<P>PROBLEM TO BE SOLVED: To provide a procedure capable of improving separation performance and detection sensitivity by suppressing increase of a peak width in a separation column following separation in liquid chromatography and electrochromatography. ??<P>SOLUTION: In this chromatography, increase of the peak width can be suppressed by combining a chromatography gradient (uneven field) column with a gradient elution procedure of a moving phase composition. ??<P>COPYRIGHT: (C)2010,JPO&INPIT ??