The technical field of the present invention is cosmetic products. Particularly, disclosed is a manufacturing method of a colored bio cellulose sheet for a makeup. The manufacturing method of the present invention can easily manufacture bio cellulose sheet for a makeup in a large quantity, and can easily adjust the width of the bio cellulose sheet for a makeup. Also, the manufacturing method of colored bio cellulose sheet uses natural substances. Thus, the manufactured sheet according to the manufacturing method does not have side effects on human bodies. The manufacturing method of the colored bio cellulose sheet comprises the following steps of: producing bio cellulose having a thickness of 0.5-2.0 mm; dehydrating and stacking 50-200 sheets of the obtained bio cellulose in a mesh bag for compression; adjusting the thickness of the sheets to be 0.1-0.6 mm; coloring the bio cellulose sheets; and cleaning and treating the colored bio cellulose sheets with preservatives.본 발명은 화장품 분야이며, 특히 화장용 칼라 바이오셀룰로오스 시트 제조방법을 개시한다. 본 발명의 제조 방법은 화장용 바이오셀룰로오스 시트의 동시 대량생산 및 두께조절이 용이하며, 천연물질을 이용하여 착색한 바이오셀룰로오스 시트 제조방법이므로 이에 따라 제조된 시트는 인체에 부작용이 없다.