Provided is a manufacturing method of propolis isolate for inhibiting cancer gene, which comprises: a propolis pretreatment step for uniformly mixing propolis (X) and ethanol (Y) in a specific ratio, placing the mixture in a centrifuge for isolation, and taking out the propolis ethanol solution of supernatant liquid after the centrifugal isolation is completed and a supercritical extraction step for obtaining the propolis isolate collected from the bottom of a supercritical chromatography column after performing a supercritical fluid extraction conditions on the propolis ethanol solution obtained in the aforementioned step under a specific supercritical fluid extraction condition. The propolis isolate is capable of regulating the expression profile of at least any one cancer gene in the gene group consisting of AQP8, CA7, IL6R, TCN1, CPEB4, CDCA3, LMNB1, and PLOD2.一種抑制癌發基因的蜂膠分離物之製備方法,其包含:將蜂膠(X)與乙醇(Y)以特定比例加以混合均勻後,放置於離心機進行分離,於離心分離結束後取出上澄液之蜂膠乙醇溶液之蜂膠前處理步驟;及以特定之超臨界流體萃取條件,對於上述所得到的蜂膠乙醇溶液進行超臨界流體萃取後,從超臨界層析管柱之底部收集而得到蜂膠分離物之超臨界萃取步驟;該蜂膠分離物係能夠調節AQP8、CA7、IL6R、TCN1、CPEB4、CDCA3、LMNB1及PLOD2的基因群組中之至少任一種癌發基因之表現量。無。
