The cold plasma sterilization device for the sterilization of at least one object is placed in a container enclosing at least a gas or a gas mixture suitable for generating a cold plasma. The device includes a closed vessel in which a support for receiving the container is arranged. There is a device for subjecting the vessel to a secondary vacuum of the order of 10−3 to 10−7 millibar. There is an electron cyclotron resonance generator for generating, in the vessel, a flow of microwaves coupled to a magnetic field at least in a phase of initialization of the cold plasma. There is also a device for polarization of the support of the container.
