CHEN, Fuqiang,DAVIS, Gregory D.,KANG, Qiaohua,KNIGHT, Scott W.
申请号：
HK16106396.9
公开号：
HK1218389A
申请日:
2016.06.06
申请国别(地区):
HK
年份:
2017
代理人:
摘要：
The present invention provides RNA-guided endonucleases, which are engineered for expression in eukaryotic cells or embryos, and methods of using the RNA-guided endonuclease for targeted genome modification in in eukaryotic cells or embryos. Also provided are fusion proteins, wherein each fusion protein comprises a CRISPR/Cas-like protein or fragment thereof and an effector domain. The effector domain can be a cleavage domain, an epigenetic modification domain, a transcriptional activation domain, or a transcriptional repressor domain. Also provided are methods for using the fusion proteins to modify a chromosomal sequence or regulate expression of a chromosomal sequence.
