Methods for producing primate cardiovascular progenitor cells for clinical use from primate embryonic stem cells or embryoid state cells and their applications

• 收藏
• 分享

专利权人:

アシスタンス ピュブリック－オピト ド パリ;ユニベルシテ、パリ、デスカルテ;アシスタンス ピュブリック-オピト ド パリ;アンスティテュ、ナショナル、ド、ラ、サント、エ、ド、ラ、ルシェルシュ、メディカル（アンセルム）;ユニベルシテ パリ ディドロ－パリ ７

发明人:

フィリップ、メナシュ,ミシェル、ピュスア,ジェローム、ラルゲロ,ギョーム、ブラン,ダビド、ヌリー,ソニア、ステファノビク

申请号：

JP2010550178

公开号：

JP5754026B2

申请日:

2009.03.10

申请国别(地区):

JP

年份:

2015

代理人:

摘要：

The present invention is directed to a method for the in vitro preparation of cardiac progenitors cells from primate embryonic stem cells (ES cells) wherein said method comprises the use of the CD15 (SSEAI) marker as a positive cardiac progenitors differentiation marker. The present invention also claimed the use of a receptor tyrosine kinase inhibitor, particularly the SU5402 or SU11248 in association with the BMP2 for improving the efficiency of the desired differentiation. The present invention is also directed to the use of platelet lysate as foetal animal serum substitute in a culture medium intended to the proliferation or propagation of primate ES cells maintaining their pluripotency feature. Derived compositions or kits in relation with the claimed methods or product obtainable by the claimed methods form also part of the present invention.

来源网站：

中国工程科技知识中心

来源网址：

http://www.ckcest.cn/home/