DETERMINING METHOD FOR ANIMAL HAIR PRODUCT
- 专利权人:
- NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL & TECHNOLOGY;BOKEN QUALITY EVALUATION INSTITUTE;KANAZAWA INST OF TECHNOLOGY
- 发明人:
- KOIKE HIDEAKI,MACHIDA MASAYUKI,YAMANE RINKO,YOSHIOKA YOICHIRO,SANO MOTOAKI
- 申请号：
- JP20130211811
- 公开号：
- JP2015073478(A)
- 申请日:
- 2013.10.09
- 申请国别(地区):
- 日本
- 年份:
- 2015
- 代理人:
- 摘要：
PROBLEM TO BE SOLVED: To provide a simple and efficient method in which the species of animal hair used for an animal hair product is determined based on its gene information.SOLUTION: There is provided a determining method for animal hair product comprising: 1) a step of cutting or crushing an objective animal hair product; 2) a step of supplying the crushed sample with DNA extracting solution as buffer solution comprising reducing agent and heating it; 3) a step of degrading protein with cysteine protease; 4) a step of extracting DNA from the animal hair product, which comprises steps of denaturing, centrifuging and ultra-filtering supernatant of the reaction solution after protein degradation so that DNA is isolated; 5) a step of carrying out PCR reaction with the isolated DNA as a template, using a set of positive control primer capable of amplifying a common DNA fragment in a plurality of animal species, and a set of primers for determination capable of amplifying a DNA fragment specific for an object
- 来源网站：
- 中国工程科技知识中心
