Patent No. 593986 Disclosed is a composition comprising a biologically active protein which is not insulin nor vascular endothelial growth factor (VEGF) and a positively charged carrier present in an effective amount for transdermal delivery, the positively charged carrier comprising a positively charged polymeric backbone having attached thereto amino acid sequences selected from the group consisting of -(gly)nl-(arg)n2, (gly)p-RGRDDRRQRRR-(gly)q , (gly)p-YGRKKRRQRRR-(gly)q, (gly)p-RKKRRQRRR-(gly)q and Antennapedia protein transduction domain (PTD)； wherein the subscript n1 is an integer of from 0 to 20, and the subscript n2 is independently an odd integer of from 5 to 25, wherein the subscripts p and q are each independently an integer of from 0 to 20, wherein the positively charged polymeric backbone is a polypeptide that promotes transdermal delivery of the biologically active protein； wherein the biologically active protein is not modified by covalent attachment to a negatively charged backbone； and wherein the positively charged carrier and the biologically active protein directly contact to form a non-covalent complex.
