The present creation relates a multi-layered Hyaluronic acid (HA) artificial skin, comprising: a HA spongy wound dressing without crossing-linking agent of releasing layer and a HA spongy wound dressing with crossing-linking agent of retaining layer. The HA releasing layer can release oligosaccharide HA in early stages of wound dressing to promote cell proliferation and migration and to enhance the angiogenesis. The HA retaining layer can retain HA and release in later stages of wound dressing to reduce the scar tissue formation and enhance the epithelization. The present creation can be applied in the patients with diabetic ulcer.本創作提供一種多層次玻尿酸人工皮膚,包含:一不具交聯試劑之玻尿酸釋放層及一具交聯劑之玻尿酸保留層,其中該玻尿酸釋放層在傷口癒合的初期釋放出寡糖-玻尿酸有助於細胞分化、細胞移動及加強血管形成;該玻尿酸保留層為可以保留玻尿酸在傷口癒合的最後階段釋放出,有助於減少組織傷口及促進上皮細胞形成。本創作適用於本糖尿病傷口潰瘍之患者之創傷治癒。10‧‧‧多層次玻尿酸人工皮膚結構101‧‧‧保護層102‧‧‧玻尿酸保留層103‧‧‧玻尿酸釋放層104‧‧‧玻尿酸保留層之第一表面105‧‧‧玻尿酸保留層之第二表面106‧‧‧玻尿酸釋放層之第三表面107‧‧‧玻尿酸釋放層之第四表面
