An assembled negative pressure isolation cabin includes a plurality of frames and isolation covers. A plurality of frame systems are adjacent to each other side by side. Each frame body has two opposite side support columns and a top column transversely connecting the side support columns. The adjacent frame bodies are pivotally connected with opening and closing parts, and the opening and closing parts are adjacent The frame can be folded or unfolded. The cover system of the isolation cover is arranged around a plurality of frames to form an isolation space. The closed entrance and exit of the isolation cover block the isolation space and the external space outside the cover. The external connection device of the isolation cover connects the air inlet device and the negative pressure device , Driven by the negative pressure device to allow the air provided by the air intake device to ventilate in the isolated space, and to form a negative pressure state in the isolated space. This creation can quickly assemble the required number of negative pressure isolation cabins with independent isolation spaces in response to demand.一種組裝式負壓隔離艙,包括複數個框體以及隔離罩。複數個框體係為並排相鄰接,各框體具有兩相對之側支撐柱、橫向連接側支撐柱之頂柱,相鄰框體之間係樞接開合件,開合件係將相鄰框體得以收合或展開。隔離罩之罩體係圍繞設置於複數個框體之周圍以構成隔離空間,隔離罩之密合出入口係阻隔隔離空間與罩體外的外部空間,隔離罩之外接裝置係連接進氣裝置與負壓裝置,由負壓裝置驅動以使進氣裝置所提供之空氣在隔離空間內進行換氣,且讓隔離空間內形成負壓狀態。本創作能夠因應需求快速組裝所需數量的獨立隔離空間之負壓隔離艙。