Bruce Stillman,Scott W. Lowe,Anthony Mazurek,Johannes Ekkehart Zuber,Christopher Vakoc,Katherine McJunkin
申请号：
US13260540
公开号：
US08895526B2
申请日:
2010.03.29
申请国别(地区):
US
年份:
2014
代理人:
摘要：
Provided is a mosaic mouse model for use in determining the potency of an shRNA in vivo for reducing survival of cancer cells of chemotherapy-resistant leukemia. The syngeneic mouse recipient is transplanted with tet-on competent leukemia cells carrying a bicistronic nucleic acid construct comprising a promoter operably linked to a fusion gene associated with chemotherapy-resistant leukemia, and a sequence encoding a reverse tet-transactivator protein, such that both coding sequences are co-expressed from the promoter. Also provided are methods of treating soft tissue cancers.
