Bruce STILLMAN,Scott W. LOWE,Anthony MAZUREK,Johannes Ekkehart ZUBER,Christopher VAKOC,Katherine MCJUNKIN
申请号：
US14531669
公开号：
US20150283101A1
申请日:
2014.11.03
申请国别(地区):
US
年份:
2015
代理人:
摘要：
Provided is a mosaic mouse model for use in determining the potency of an shRNA in vivo for reducing survival of cancer cells of chemotherapy-resistant leukemia. The syngeneic mouse recipient is transplanted with tet-on competent leukemia cells carrying a bicistronic nucleic acid construct comprising a promoter operably linked to a fusion gene associated with chemotherapy-resistant leukemia, and a sequence encoding a reverse tet-transactivator protein, such that both coding sequences are co-expressed from the promoter. Also provided are methods of treating soft tissue cancers.
