A cultivation method of North Cordyceps sinensis, comprises the following steps: step 1: purifying bacteria; step 2: producing bacteria; step 3: producing nutrient solution; step 4 producing solid culture material; step 5: cultivating mycelia; step 6: prompting color transfer of the mycelial; step 7: completing the cultivation of Cordyceps militaris. By following these steps, the simple and stable mass production of Cordyceps militaris can be guaranteed, which is an cost-effective method for cultivating Cordyceps militaris.一種北冬蟲夏草的培育方法,包含有下列步驟:步驟一:純化菌種;步驟二:生產種菌;步驟三:營養液配置;步驟四:固體培養料配置;步驟五:培養菌絲;步驟六:促使該菌絲轉色;步驟七:完成北冬蟲夏草的培育。並藉由以上步驟,達到簡易且可穩定批次北冬蟲夏草子實體產量以及符合成本效益的北冬蟲夏草的培育方法。