Is to provide an anti-hair graying agent object of the present invention. Method characterized in that there is provided a method of screening anti-hair graying agent, selecting a drug is applied to cells in vitro a candidate agent, enhancing the expression of AFF-4 in the cell, by the method according to the arrangement to the invention the extract of crude drug of at least one kind selected from the group consisting of (Rabdosia japonicus), carrots (Panax schinseng Nees), rice bran cause (Oryza Sativa (Rice) Bran) and ((Fr.) Karst. Ganoderma lucidum) Reishi that have been selected , to provide an anti-hair graying agent containing an effective amount of the enhanced expression of the AFF-4.本発明の目的は抗白髪剤の提供にある。本発明は抗白髪剤のスクリーニング方法であって、候補薬剤をin vitroで細胞に適用し、当該細胞のAFF-4の発現を亢進させる薬剤を選定することを特徴とする方法、並びにかかる方法により選定されたレイシ(Ganoderma lucidum (Fr.) Karst.)、ニンジン(Panax schinseng Nees)、コメヌカ(Oryza Sativa (Rice) Bran)及びヒキオコシ(Rabdosia japonicus)からなる群から選ばれる少なくとも一種の生薬のエキスを、AFF-4の発現亢進に有効量で含有する抗白髪剤を提供する。
