Provided are a small interfering nucleic acid against bone formation inhibiting gene CKIP-1, a pharmaceutical composition thereof, and uses thereof in preparation of a pharmaceutical composition for treating and/or preventing diseases related to the abnormal expression of CKIP-1 gene. The small interfering nucleic acid is capable of cross-species inhibiting the CKIP-1 gene expression, inhibiting CKIP-1 expression in human, rhesus, rats and mice simultaneously, and facilitating the differentiation of osteoblasts and mineralization of bone matrix effectively.
