WILSON, JAMES M.,BELL, CHRISTIE L.,VANDENBERGHE, LUC H.
申请号：
CA2826316
公开号：
CA2826316A1
申请日:
2012.02.17
申请国别(地区):
CA
年份:
2012
代理人:
摘要：
It is a kind of change targeting and/or cellular uptake efficiency gland satellite virus (AAV) viral vector have capsid include AAV9 cell surfaces bond area be described. This method is related to modifying clade F cell surface receptors including the polysaccharide residue that glycan has terminal sialic acid residue and penultimate beta galactose. The modification can be related to the carrier and temporarily functionally be removed AAV9 blocking unit subsets again, and the carrier is thus directed toward another cell subsets again. Optionally, which, which can be related to increasing honeycomb, updates efficiency, by with neuraminic acid enzymatic treatment cell, with the polysaccharide of exposed cell surface beta galactose. Composition is additionally provided, AAV9 vector sum neuraminidases are contained. It also provides and a kind of is attached to support using the polysaccharide of beta galactose for purifying AAV9. Additionally providing these mutant vectors is the modified target step to change them, including wherein wherein AAV9 galactolipins binding structural domain is engineered the mutation of galactolipin binding structural domain mutant AAV9 with AAV.
