[Problem] To provide: a method for breeding a novel species by interspecies crossing among Pleurotus sp. varieties such as Pleurotus eryngii (DC. :Fr.) Quel, Pleurotus eryngii (DC. :Fr.) Quel. var. elaeoselini, Pleurotus eryngii (DC. :Fr.) Quel. var. tuoliensis C. J. Mou and Pleurotus nebrodensis (DC. :Fr.) Quel which are not native in Japan and Pleurotus ostreatus (Jacq: Fr.) Kummer which is native in Japan; and a novel species produced by the method. [Solution] Pleurotus ostreatus (Jacq: Fr.) Kummer is crossed with Pleurotus eryngii (DC. :Fr.) Quel. var. tuoliensis C. J. Mou, Pleurotus nebrodensis (DC. :Fr.) Quel or Pleurotus eryngii (DC: Fr.) Quel. var. ferulae Lanzi, whereby a strain capable of further crossing with Pleurotus eryngii (DC. :Fr.) Quel, Pleurotus eryngii (DC. :Fr.) Quel. var. elaeoselini or the like can be produced. Thus, a novel species of Pleurotus sp. which carries both a Pleurotus eryngii (DC. :Fr.) Quel gene and a Pleurotus ostreatus (Jacq: Fr.) Kummer gene can be provided.