Provided are an antibody for sexing sperms and use thereof, and more particularly, a composition for and a method of sexing sperms by using the antibody, and a method of producing an animal of a particular sex. In the present disclosure, it was confirmed that agglutination of Y chromosome sperms may be induced by treatment of the antibody, thereby easily sorting X chromosome sperms and Y chromosome sperms. Therefore, it is possible to produce a large number of customized animals of a particular sex and to selectively produce livestock of a desired sex, and therefore, it is expected to contribute to planed breeding, breeding improvement, and efficient management.
