A light emitting surgical calibration probe device includes a first optical tracking assembly, a quick disconnect coupler, a second positioning assistance unit, and a probe. The first optical tracking assembly has at least one tracking markers on a surface, and a surgical navigation system converts the position of the tracking markers into an overall position. The quick disconnect coupler is detachably coupled to the first optical tracking assembly and the second positioning assistance unit, and the second positioning assistance unit is capable of emitting an indicating light source with a specific wavelength and provided for a surgical navigation system to perform a non-contact positioning function. The probe is movably coupled to the second positioning assistance unit. The first optical tracking assembly and the second positioning assistance unit allow doctors to know the position of a surgical robot with respect to a patient's trackable housing more accurately.