Disclosed is an anti-bacterial composition using essential oils of retinispora, Chrysanthemum indicum, Dendrathema boreale, summer chrysanthemum, peppermint, Chinese pepper, Hemerocallis fulva, and/or Japanese cinnamon. The anti-bacterial composition of the present invention can be used in medicine, cosmetics, household goods, and food in an antibacterial purpose, can be manufactured in gas phase such as spray formulations, or can be used in the form of synthetic fibers or natural fiber materials impregnated with a hygroscopic medium.COPYRIGHT KIPO 2014본 발명은 편백나무 정유 추출물 및/또는 감국, 산국, 쑥갓, 박하, 산초, 원추리 및 육계 혼합물의 정유 추출물을 이용한 항균 조성물을 개시한다.