PROBLEM TO BE SOLVED: To provide a biological tissue adhesive applicator that can satisfactorily prevent its tip from being solidified under intermittent use.SOLUTION: The biological tissue adhesive applicator 100 includes: a nozzle body 1; a gas injection port 2; chemical solution injection ports 3a, 3b; chemical solution discharge ports 4a, 4b; chemical solution circulation tubes 5a, 5b serving for communication between the chemical solution injection ports 3a, 3b and chemical solution discharge ports 4a, 4b respectively; a gas jet port 6; two communicating ports 8a, 8b provided at the chemical solution circulation tube 5b to introduce gas inside the nozzle body 1 toward the chemical solution discharge port 4b; and check valves 7a, 7b provided at the chemical solution circulation tubes 5a, 5b to allow a chemical solution to flow into the chemical solution circulation tubes 5a, 5b from the chemical solution injection ports 3a, 3b and to regulate the outflow of the chemical solution toward the chemical solution discharge ports 4a, 4b from the inside of the chemical solution circulation tubes 5a, 5b.【課題】断続的な使用時における先端の固化を良好に防止できる生体組織接着剤塗布用具を提供する。【解決手段】生体組織接着剤塗布用具100を、ノズル本体1と、ガス注入口2と、薬液注入口3a、3bと、薬液吐出口4a、4bと、薬液注入口3a、3b及び薬液吐出口4a、4bを各々連通する薬液流通管5a、5bと、ガス噴出口6と、薬液流通管5bに設けられ、ノズル本体1内部のガスを薬液吐出口4b方向に導入する2つの連通口8a、8bと、薬液流通管5a、5bに設けられ、薬液注入口3a、3bから当該薬液流通管5a、5b内部への薬液の流入を許容し、当該薬液流通管5a、5b内部からの薬液吐出口4a、4b方向への薬液の流出を規制する逆止弁7a、7bと、を有して構成する。【選択図】図1