이옥환,이영준,윤보라,이대원,김명동,LEE, OK HWAN,LEE, YOUNG JUN,YOON, BO RA,LEE, DAE WON,KIM, MYOUNG DONG
申请号：
KR1020120084841
公开号：
KR1012030960000B1
申请日:
2012.08.02
申请国别(地区):
KR
年份:
2012
代理人:
摘要：
PURPOSE: A method for preparing fermented Artemisia princeps and pine needle extract is provided to reduce fat accumulation and to suppress ROS generation. CONSTITUTION: A method for preparing fermented medicinal herb extract comprises a step of inoculating lactic acid bacteria to Artemisia princeps and pine needle extract with the lactic acid bacteria and fermenting. The lactic acid bacteria are Leuconostoc mesenteroides 1076. The extract is prepared by pulverizing Artemisia princeps and pine needles, adding distilled water, and extracting at 24-26 Deg. C. for 23-25 hours. The method comprises: a step of pulverizing one of pine needles or Artemisia princeps； a step of adding water and extracting at 25 Deg. C. for 24 hours； a step of placing the extract at 0-4 Deg. C.； a step of inoculating Leuconostoc mesenteroides 1076 isolated from Kimchi； a step of fermenting at 30 Deg. C. and 200 rpm for 36 hours and centrifuging； a step of freeze-drying at 40 Deg. C. [Reference numerals] (AA) Selecting Artemisia princeps and pine needles； (BB) Washing and drying； (CC) Pulverizing (20-30 mesh)； (DD) Incubating (0-4°；C,24hr)； (EE) Low temperature extraction (25°；C,24hr)； (FF) Adding 10 times of D.W； (GG) Inoculating strain into medicinal herb extract； (HH) Preculture of Leuconostoc mesenteroides 1076； (II) Selecting excellent strains with mannitol high productivity； (JJ) Fermentation process(30°；C, 200rpm, 36 hr)； (KK) Centrifuging(13,200 rpm)； (LL) concentration at reduced pressure(40°；C)； (MM) Pulverizing； (NN) Freeze-drying본 발명은 젖산균을 이용하여 발효시켜 생리활성이 증진된 발효 쑥 및 발효 솔잎 추출물의 제조방법을 제공한다.
