Nucleic acid hybridization methods are disclosed. An example method comprises: immobilizing probe nucleic acid molecules on a surface; flowing target nucleic acid molecules to the immobilized probe nucleic acid molecules on said surface in a hybridization buffer solution; washing said surface with a wash solution which comprises nanoparticles; and detecting the presence of duplexes on said surface comprising a strand of one of said target nucleic acid molecules and a strand of one of said probe nucleic acid molecules. In some embodiments, the target nucleic acid molecules are generated using a helicase-dependent amplification method wherein the reaction solution comprises nanoparticles.
