Beihao Stem Cell and Regenerative Medicine Translation Research Institute
发明人:
DENG, Hongkui,DU, Yuanyuan,SHI, Yan,JIA, Jun,WANG, Jinlin,XIANG, Chengang,SONG, Nan,XU, Jun,YIN, Ming
申请号：
EP15748953
公开号：
EP3105315A4
申请日:
2015.02.04
申请国别(地区):
EP
年份:
2017
代理人:
摘要：
A method for inducing reprogramming of a cell of a first type which is not a non-hepatocyte (non-hepatocyte cell), into a cell with functional hepatic drug metabolizing and transporting capabilities, is disclosed. The non-hepatocyte is induced to express or overexpress hepatic fate conversion and maturation factors, cultured in somatic cell culture medium, hepatocyte cell culture medium and hepatocyte maturation medium for a sufficient period of time to convert the non-hepatocyte cell into a cell with hepatocyte-like properties. The iHeps induced according to the methods disclosed herein are functional induced hepatocytes (iHeps) in that they express I and II drug-metabolizing enzymes and phase III drug transporters and show superior drug metabolizing activity compared to iHeps obtained by prior art methods. The iHeps thus provide a cell resource for pharmaceutical applications.
