BeiHao Stem Cell and Regenerative Medicine Translational Research Institute;Beijing Vitalstar Biotechnology Co., Ltd.;Peking University
发明人:
Deng, Hongkui,Du, Yuanyuan,Shi, Yan,Jia, Jun,Wang, Jinlin,Xiang, Chengang,Song, Nan,Xu, Jun,Yin, Ming
申请号：
AU2015218082
公开号：
AU2015218082A8
申请日:
2015.02.04
申请国别(地区):
AU
年份:
2016
代理人:
摘要：
A method for inducing reprograming of a cell of a first type which is not a non-hepatocyte (non-hepatocyte cell), into a cell with functional hepatic drug metabolizing and transporting capabilities, is disclosed. The non-hepatocyte is induced to express or overexpress hepatic fate conversion and maturation factors, cultured in somatic cell culture medium, hepatocyte cell culture medium and hepatocyte maturation medium for a sufficient period of time to convert the non-hepatocyte cell into a cell with hepatocyte-like properties. The iHeps induced according to the methods disclosed herein are functional induced hepatocytes (iHeps) in that they express I and II drug-metabolizing enzymes and phase III drug transporters and show superior drug metabolizing activity compared to iHeps obtained by prior art methods. The iHeps thus provide a cell resource for pharmaceutical applications.
