L The present invention relates to a method for tissue culture of Ornithogalum caudatum, including the following steps: medium preparation, explant selection and sterilization, primary culture, adventitious bud induction, adventitious bud multiplication, adventitious bud rooting induction, tissue culture seedling domestication and transplantation. The minimal media select MS media, where sucrose is used in an amount of 20—40 g/L, and agar powder, which is used as the coagulating agent, is used in an amount of 8—9 g/L, and the pH of the media is adjusted to 5.7—5.8 10 before subpackaging; the medium for primary culture is MS+6—BA 0.1—0.5 mg/L+NAA 0.05—O.2 mg/L; the medium for adventitious bud induction is MS+6—BA—2.0—4.0 mg/L+NAA 0.05—0.2 mg/L; the medium for adventitious bud multiplication is MS+6—BA O.5—3.0 mg/L+NAA 0.05—0.2 mg/L; and the medium for rooting is MS+IBA 0—0.5 mg/L or 15 l/2MS+IBA 0—0.5 mg/L.
