Proposed invention is related to the field of photodynamic therapy and photodiagnosis. Specifically, a new algorithm is presented for visualisation and spatial demarcation of various types of tumours and unhealthy tissue through unsupervised segmentation of the fluorescent multispectral image. Image is acquired through recording of the emission of the tissue illuminated by the light that induces fluorescence in the tumour. For this purpose tissue is treated by photo sensitizer. Algorithm for real time visualisation and spatial demarcation of the tumour by means of the analysis of fluorescent image consists of the recording of the fluorescent image (1) that represents linear combination of the unknown classes S by multispectral camera, transformation of the fluorescent multispectral image into new multispectral image (2) applying nonlinear dimensionality expansion, linear transformation of the multispectral image (2) with extended dimensionality, independent component analysis of the innovation representations of the multispectral image (3) that is result of the transformation of the fluorescent image (1) and multispectral image (2), extraction o the tumour map or class of interest in accordance with the chosen criterion and visualisation of tumour on monitor.Linvention concerne le domaine de la thérapie photodynamique et du photodiagnostic. De manière spécifique, un nouvel algorithme est présenté, pour une visualisation et une démarcation spatiale de divers types de tumeur et de tissu non sain par une segmentation non supervisée de limage multispectrale fluorescente. Limage est acquise par lenregistrement de lémission du tissu éclairé par la lumière qui induit une fluorescence dans la tumeur. A cette fin, le tissu est traité par un photo-sensibilisateur. Un algorithme pour une visualisation en temps réel et une démarcation spatiale en temps réel de la tumeur au moyen de lanalyse dune image fluorescente consiste en lenregistrement de limage fluorescente (1) qui repré
