The invention relates to a method, a guide assembly and a system for separating poultry viscera sets (1) suspended from a viscera holder (2). In the method a guide member (4) is moved so that said viscera set (1) is arranged in an elongated opening (43) of the guide member with the intestines and the gall- bladder suspended below said guide member and the liver (16) and gizzard (17) positioned above it. The intestines (15) and the gallbladder are then separated from the remaining viscera set (1) while said liver (16) rests on a first part (41) of a separation support surface and said gizzard (17) on a second part (42) on the opposite side of an elongated opening. Before the separation, the viscera set (1) is brought into contact with an engagement member (8) so that the gizzard (17) is kept on the one side of the elongated opening (43) at least until coming into contact with the separation support surface (41).
