The present invention relates to a method for preparing a bacterial supernatant comprising culturing cells of Pseudomonas environmental strain PF-11 and recovering the supernatant. The invention also provides a method for reducing the amount of biofilm on a surface, reducing adhesion of at least one organism to the surface, or reducing surface microfouling or macrofouling, comprising Pseudomonas. ) Strain PF-11 supernatant, supernatant fraction, modified supernatant, or modified supernatant fraction, or Pseudomonas strain PF-11 supernatant, supernatant fraction, modified supernatant, or modified supernatant It relates to a method comprising contacting a surface with a composition comprising a liquid fraction and one or more acceptable carriers. The invention also relates to a method of killing fungi or bacterial cells or reducing their growth or killing insects or marine copepods or inhibiting their development, comprising a Pseudomonas strain PF-11 culture A supernatant, a supernatant, a modified supernatant, a modified supernatant, or a modified supernatant, or a Pseudomonas strain PF-11 culture supernatant, supernatant fraction, modified supernatant, or modified supernatant. It relates to a method comprising contacting a composition comprising a fraction and one or more acceptable carriers with a fungus, bacterium, insect, or marine copepod. The present invention also relates to a substantially pure culture of Pseudomonas strain PF-11. The present invention also relates to a culture enriched for Pseudomonas strain PF-11. The present invention also provides a method for identifying whether a bacterium is capable of producing one or more extracellular proteases capable of digesting a high molecular weight substrate.本発明は、シュードモナス（Ｐｓｅｕｄｏｍｏｎａｓ）環境株ＰＦ－１１の細胞を培養することと、上澄み液を回収することとを含む、細菌上澄み液を調製する方法に関する。本発明はまた、表面のバイオフィルムの量を減少させる、少なくとも１種の有機体が表面へ付着するのを減少させる、または表面のマイクロ汚損またはマクロ汚損を減少させる方法であって、シュードモナス（Ｐｓｅｕｄｏｍｏｎａｓ）株ＰＦ－１１の上澄み液、上澄み液分画、修飾上澄み液、もしくは修飾上澄み液分画、またはシュードモナス（Ｐｓｅｕｄｏｍｏｎａｓ）株ＰＦ－１１の
