The present invention provides a method for producing Pseudomonas sp. PF-11, comprising culturing cells of Pseudomonas sp. PF-11； And recovering the supernatant. The present invention also relates to a method for producing a bacterial supernatant. The present invention also relates to the use of a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of Pseudomonas sp. PF-11 on a surface； Or a composition comprising Pseudomonas sp. PF-11, a supernatant, a supernatant fraction, a modified supernatant or a modified supernatant fraction, and one or more acceptable carriers, to reduce the amount of biofilm on the surface, Consideration is given to reducing the adhesion of at least one organism to the surface, or reducing fine deposits or giant deposits on the surface. The present invention also relates to the use of a supernatant, a supernatant fraction, a modified supernatant or a modified supernatant fraction of a Pseudomonas strain PF-11 culture, a fungal, bacterial, insect or marine copepod； Or Pseudomonas sp. PF-11 culture supernatant, supernatant fraction, modified supernatant or modified supernatant fraction, and one or more acceptable carriers. Inhibit, and kill insects or marine copepods or inhibit their occurrence. The present invention also contemplates a substantially pure culture of Pseudomonas sp. PF-11. The present invention also contemplates cultures enriched with Pseudomonas sp. PF-11. The present invention also provides a method for determining whether a bacterium is capable of producing one or more extracellular proteases capable of degrading a high molecular weight substrate.본 발명은 슈도모나스 환경 균주 PF-11의 세포를 배양하는 단계； 및 상청액을 회수하는 단계를 포함하는 세균 상청액을 제조하는 방법에 관한 것이다. 본 발명은 또한 표면을 슈도모나스 균주 PF-11의 상청액, 상청액 분획, 변형된 상청액 또는 변형된 상청액 분획； 또는 슈도모나스 균주 PF-11의 상청액, 상청액 분획, 변형된 상청액 또는 변형된 상청액 분획, 및 하나 이상의 허용가능한 담체를 포함하는 조성물과 접촉시키는 단계를 포함하는, 표면 위에 생물막의 양을 감소시키고, 표면에 대한 적어도 하나의 유기체의 부착을 감소시키고, 또는 표면 위에 미세부착물 또는 거대부착물을 감소시키는 방법을 고려한다. 본 발
