Disclosed is a gas chromatography chip including: a substrate having an upper substrate and a lower substrate; a gas supply connecting part formed on one plane of one surface of the substrate or on one plane of an opposite surface of the substrate; a gas discharge connecting part formed another plane of the one surface of the substrate or on another plane of the opposite surface of the substrate; a micro channel continuously extending from the gas supply connecting part to the gas discharge connecting part to form a micro channel part and having a circular cross-section; at least two position alignment markers formed on one surface or an opposite surface of the opposite surfaces of the substrate; and a heat transfer part and a temperature control unit for controlling a temperature of the substrate.
