PURPOSE: A method of increasing transgene expression in a population of monocot plant cells is provided which involves the use of a DNA construct comprising, inter alia, at least one chicken lysozyme gene locus scaffold attachment region. CONSTITUTION: A method comprises: (a) transforming the population with a DNA construct which comprises: (1) a transgene comprising, in the 5' to 3' direction: (i) a promoter; (ii) a coding sequence operably linked to the promoter, and (iii) a polyadenylation signal sequence operably linked to the coding sequence; and (2) at least one chicken lysozyme gene locus scaffold attachment region wherein the scaffold attachment region is positioned 5', 3', or 5' and 3' of the transgene; and (b) incubating the transformed population under conditions suitable for cell growth.
