The invention concerns a method for the preparation of an acellular organic tissue for revitalization, in particular for the introduction of living cells, comprising a stage in which the acellular organic tissue (2 12) is provided with a plurality of holes (4 14) made through its surface (8 18) and extending towards the inside of the tissue (2 12). The holes intersect partially, thus forming holes (4 14) partially communicating with each other. The invention also concerns the acellular organic tissue obtained with this method.
