The invention concerns a method for the preparation of an acellular organic tissue of human or animal origin for revitalization in particular for the introduction of living cells comprising a stage in which the acellular organic tissue (2 12) is provided with a plurality of holes (4 14) made through its surface (8 18) and extending towards the inside of the tissue (2 12) wherein the plurality of holes (4 14) is made by means of one or more needles. The holes (4 14) intersect partially thus forming holes (4 14) partially communicating with each other.
