Chung-Ang University Industry-Academy Cooperation Foundation
发明人:
KANG, Hyun Ah,MOON, Hye Yun,KIM, Hong Jin
申请号：
EP20140900237
公开号：
EP3184639(A1)
申请日:
2014.12.30
申请国别(地区):
欧洲专利局
年份:
2017
代理人:
摘要：
The present invention relates to a ribosomal DNA nontranscribed spacer (rDNA NTS)-based gene multiple insertion cassette set and a GRAS-grade recombinant yeast strain using the same. In particular, a gene multiple insertion cassette set including rDNA NTS fragments and an auxotrophic selection marker having an incomplete promoter is developed, and a safe oral recombinant strain having no antibiotic resistant marker is constructed by multiple insertion of an optimum number of the developed gene multiple insertion cassette sets into chromosomes of a Saccharomyces cerevisiae strain. In addition, the present invention provides a vaccine composition including, as an active ingredient, the above strain, a culture product thereof, a cell lysate, or nodavirus capsid protein (NNVcp) isolated and purified therefrom. In addition, the present invention provides a composition for feed addition including, as an active ingredient, the above strain, a culture product thereof, a cell lysate, or squalene or oxidosqualene iso
