A sample holder for provocation sterilization tests (10) comprising: a body (12) defining an internal chamber zone (36), which has an internal wall (37)； a cover (14) that can be sealed at a first end (22) of the body (12)； a pin (16) placed at a second end of the body (12) and having an opening (62) formed inside which at least partially defines a single tortuous entry and exit path (44) formed as a groove in the form of spiral that communicates the internal chamber (36) with the surroundings (E), the plug (16) that has an outer wall (46) and that provides an external seal (50) between the environments (E) and the inner wall of the body (37)； and an insulating layer (18, 218) on at least a part of the body (12) and the cover (14) to isolate the body (12) and the cover (14) but not above the opening (62) so that does not interfere with the input and output path (44), the insulating layer placed on the plug to keep the opening in communication with the environments, reducing the insulating layer (18, 218, 618) heat transfer from the environments (E ) to the internal chamber zone (36)； wherein the spiral groove (44) is formed in an inner wall of the body (37) and the outer wall (46) of the plug.Un portamuestras para pruebas de esterilización por provocación (10) que comprende: un cuerpo (12) que define una zona de cámara interna (36), el cual tiene una pared interna (37)； una tapa (14) que puede sellarse en un primer extremo (22) del cuerpo (12)； una clavija (16) colocada en un segundo extremo del cuerpo (12) y que tiene una abertura (62) formada en su interior que define al menos parcialmente una única vía de entrada y salida tortuosa (44) formada como una ranura en forma de espiral que comunica la cámara interna (36) con los entornos (E), la clavija (16) que tiene una pared exterior (46) y que proporciona un sellado externo (50) entre los entornos (E) y la pared interior del cuerpo (37)； y una capa aislante (18, 218) sobre al menos una parte del cuerpo (12) y la tap
