THE MOUNT SINAI SCHOOL OF MEDICINE OF NEW YORK UNIVERSITY
发明人:
GARCIA-SASTRE, ADOLFO,PALESE, PETER
申请号：
DK10012455
公开号：
DK2336370T3
申请日:
1999.09.14
申请国别(地区):
DK
年份:
2016
代理人:
摘要：
This invention relates to genetically engineered Newcastle disease viruses and viral vectors which express heterologous genes or mutated Newcastle disease viral genes or a combination of viral genes derived from different strains of Newcastle disease virus. The invention relates to the construction and use of recombinant negative strand NDV viral RNA templates which may be used with viral RNA-directed RNA polymerase to express heterologous gene products in appropriate host cells and/or to rescue the heterologous gene in virus particles. In a specific embodiment of the invention, the heterologous gene product is a peptide or protein derived from the genome of a human immunodeficiency virus. The RNA templates of the present invention may be prepared by transcription of appropriate DNA sequences using any DNA-directed RNA polymerase such as bacteriophage T7, T3, SP6 polymerase, or eukaryotic polymerase I.
